(a) biodiesel production
(b) seedless fruit production
(c) energy production from sewage
(d) gene transfer without a vector
[CBSE AIPMT 2009]
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Aug 16, 2021
Polyethylene glycol method is used for
Polyethylene glycol method is used for
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Correct Option (d) Reason : Polyethylene glycol method is used for gene transfer without a vector. Introduction of DNA into plant cells without the involvement of a biological agent and leading to stable transformation is known as direct gene transfer. There are various methods for direct direct gene transfer, one of which is chemical method.
Certain chemicals, e.g. PEG (Polyethylene Glycol), polyvinyl alcohol and calcium phosphate enhance the uptake of DNA by plant protoplast. PEG and calcium phosphate are thought to precipitate the DNA onto the outer surface of plasmalemma and the precipitate is taken up by the endocytosis.